Genetic progress forward

2009, a busy year on all fronts

Births of the clones of Levisto, Air Jordan and Ratina. E.T. Cryozootech-Stallion and Pieraz-Cryozootech started successfully their reproductive life. And thanks to our research program, we are pleased to diversify our activity towards ICSI.

Productive year for births : 5 lovely outstanding foals, clones of Levisto, Air Jordan and above all three clones of Ratina. Those foals were born last April and arrived last July at Mr Melchior’s place.

left, the clones of Levisto,CRYOZOOTECH clone of AIR JORDAN

right, the clone of Air Jordan,


the 3 clones of Ratina


PIERAZ is now well-integrated in the endurance breeding thanks to the team of the Haras du Freysse, Claire Martin and Doctor Jean-Luc CHAMBOST, their veterinary. No less than 35 mares mated with Pieraz and 25 were pregnant end of the season. We’re eager to see all these foals at a show in 2010. Due to the good results Claire MARTIN had with him, she’ll be responsible for Pieraz’ reproduction in 2010. Conditions remain identical dernière.

Hereafter : Pieraz in Haras du Freysse, together with Pierazade and Vegaz, his first two 2008 and 2009 fillies.


Pieraz Haras du Freysse

E.T.-CRYOZOOTECH-STALLION started its reproductive life in 2009 with a few number of breedings. Due to his young age (2.5 years since born late), he covered 8 mares among which some were away, other were embryo transfer or covered late in the season. 4 of them were diagnosed full, one of them being the recipient of the famous Uélème. It’s breaking occured end of summer so that he could be presented ridden, but he won’t participate in competition, which would be against our creed : clones exist to transmit an already proven genetics.

E.T.-CRYOZOOTECH STALLION will breed in 2010 at Domaine de Laye – Aurélien Laguide (tel. : +33

Hereafter some pictures of E.T.CRYOZOOTECH STALLION during Summer 2009.

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Diversification of our activities : OPU+IVM+ICSI

Both our research team and our mares have been dedicated this year to those techniques so as to meet a real need


OPU (Ovum Pick Up) : it consists in puncturing all available follicles from a mare so as to recover the immature oocytes, having them then matured in vitro. 5 oocytes every 10 days can be obtained, which is to say 10 oocytes per cycle

IVM (In Vitro Maturation) : it enables the fertilisability of immature oocytes.

ICSI (Intra-Cytoplasmic Sperm Injection) : this type of constrained In-Vitro Fertilization via micro-manipulation enables to by-pass the low efficiency observed in the mare In-Vitro Fertilization.

Significant progress has been made and we’re close to success. Soon Cryozootech will offer his clients this technique OPU+IVM+ICSI. It gives the opportunity to fertilise infertile mares, to work with sub-fertile stallions and to fertilise several mares with only one dose of semen, which is of real interest applied to dead stallions for which the semen stock is very low.


Spermatozoon injection

In-Vitro embryo after ICSI